Benchmarking RFdiffusion generated binders for IL-7Ra

For this benchmarking study, we focused on AI-generated designs against Interleukin-7 receptor-α (IL-7Ra), a protein involved in several inflammatory and autoimmune related disorders.

In the original paper, they experimentally tested 95 binder designs for IL-7Ra and found 32 sequences that bind to the target. We took those 32 binding sequences as well as 10 randomly selected non-binders for a total of 42 designs and ran them through our Affinity Characterization workflow to see how the results hold up. You can find a detailed comparison of the experimental workflows in our repository. The entire workflow from receiving the DNA to processing the experimental data took approximately 16 hours and less than 20 minutes of manual handling.

See more detail at https://www.adaptyvbio.com/blog/rfdiff_il7ra